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Product Name
Anti-Vimentin Rabbit antibody
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Description
Vimentin Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Vimentin
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/30
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Validations
Immunofluorescent analysis of Hela cells, using Vimentin Antibody.
Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.
Western blot analysis of extracts from K562, 3T3, C6 and Hela cells using Vimentin Rabbit pAb at 1:1000 dilution. Predicted band size: 54kDa. Observed band size: 54kDa.
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Background
Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"