Anti-Vimentin Rabbit antibody

Cat.#: 167171

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Product Information

  • Product Name
    Anti-Vimentin Rabbit antibody
  • Documents
  • Description
    Vimentin Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthesized peptide derived from human Vimentin
  • Clonality
    Polyclonal
  • Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500-1/2000

    IHC: 1/50-1/200

    ICC/IF: 1/50-1/200

    FC: 1/30

  • Validations

    Immunofluorescent analysis of Hela cells, using Vimentin Antibody.

    Immunofluorescent analysis of Hela cells, using Vimentin Antibody.

    Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.

    Immunohistochemical analysis of paraffin-embedded human colon, using Vimentin Antibody.

    Western blot analysis of extracts from K562, 3T3, C6 and Hela cells using Vimentin Rabbit pAb at 1:1000 dilution. Predicted band size: 54kDa. Observed band size: 54kDa.

    Western blot analysis of extracts from K562, 3T3, C6 and Hela cells using Vimentin Rabbit pAb at 1:1000 dilution. Predicted band size: 54kDa. Observed band size: 54kDa.

  • Background
    Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"