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Product Name
Anti-Vimentin (10E2) Mouse antibody
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Description
Vimentin (10E2) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG1
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Preparation
Antigen: Recombinant Protein of Vimentin
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-3000
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Validations
Western blot detection of Vimentin in Jurkat,COS7,Hela cell lysates using Vimentin (10E2) Mouse mAb(1:2000 diluted).Predicted band size:50-57KDa.Observed band size:55KDa.
The picture was kindly provided by our customer
Western blot analysis of 293T cell with Mouse mAb diluted at 1:1000
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.
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Background
Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"