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Product Name
Anti-Vimentin (4F8) Mouse antibody
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Description
Vimentin (4F8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP, IHC-P
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Species reactivity
Human
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Alternative names
CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin. antibody
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Isotype
Mouse IgG2a
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Preparation
Antigen: Purified recombinant human Vimentin protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/800
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Validations
Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.
Immunohistochemical analysis of paraffin-embedded human tonsils using Vimentin (4F8) Mouse mAb.
Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.
Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).
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Background
Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
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References
- Repression of miR-135b-5p promotes metastasis of early-stage breast cancer by regulating downstream target SDCBPScreening of Recurrence Related MicroRNA in Ductal Carcinoma In Situ and Functional Study of MicroRNA-654-5p.
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