Anti-Vimentin (4F2E8) Mouse antibody

Cat.#: 168297

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Product Information

  • Product Name
    Anti-Vimentin (4F2E8) Mouse antibody
  • Documents
  • Description
    Vimentin (4F2E8) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P
  • Species reactivity
    Human, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of Vimentin (aa2-466) expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Western blot detection of Vimentin in U2OS,293,COS7 and Hela cell lysates using Vimentin mouse mAb (dilution 1:500).Predicted band size:54KDa.Observed band size:54KDa.

    Western blot detection of Vimentin in U2OS,293,COS7 and Hela cell lysates using Vimentin mouse mAb (dilution 1:500).Predicted band size:54KDa.Observed band size:54KDa.

  • Background
    Swiss-Prot Acc.P08670.Vimentin, also know as VIM. It is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"