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Product Name
Anti-Phospho-Vimentin (Ser56) Rabbit antibody
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Description
Phospho-Vimentin (Ser56) Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser56 of human Vimentin
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
ICC/IF: 1/200
FC: 1/20
IP: 1/20
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Validations
Western blot detection of Phospho-Vimentin (Ser56) in U251MG cell lysates using Phospho-Vimentin (Ser56) Rabbit mAb(1:500 diluted).Predicted band size:54kDa.Observed band size:54kDa.
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Background
Swiss-Prot Acc.P08670.Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"