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Product Name
Anti-Phospho-AKT (Thr450) Rabbit antibody
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Description
Phospho-AKT (Thr450) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using AKT (phospho-Thr450) Rabbit pAb (166704,1:500 diluted,upper) and AKT(pan) Mouse mAb (200323,lower).
Western blot analysis of extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using AKT1 (phospho-Thr450) Antibody .
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Background
Swiss-Prot Acc.P31749.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"