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Product Name
Anti-Phospho-AKT (Ser473) Rabbit antibody
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Description
Phospho-AKT (Ser473) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000IHC:1/50-1/100
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Validations
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Akt (Phospho-Ser473) Antibody .
Western blot analysis of extracts from 293 cells, treated with EGF or calf intestinal phosphatase (CIP), using Akt (Phospho-Ser473) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using Akt (Phospho-Ser473) Rabbit pAb (167146,1:500 diluted upper) and AKT(pan) Mouse mAb (200323,lower).
Immunohistochemical analysis of paraffin-nembedded human Lung carcinoma tissue using nAkt (Phospho-Ser473) Antibody .
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Background
Swiss-Prot Acc.P31749.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"