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Product Name
Anti-AKT Rabbit antibody
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Description
AKT Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human AKT1/2/3
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Western blot analysis of extracts from K562, A549 and Jurkat cells using AKT Rabbit pAb at 1:1000 dilution. Predicted band size: 56kDa. Observed band size: 60kDa.
Immunofluorescent analysis of MCF-7 cells, using AKT Antibody.
Immunohistochemical analysis of paraffin-embedded human testis, using AKT Antibody.
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Background
Swiss-Prot Acc.P31749,P31751,Q9Y243.Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"