Anti-phospho-AKT1 (Thr450) Rabbit antibody

Cat.#: 168055

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Product Information

  • Product Name
    Anti-phospho-AKT1 (Thr450) Rabbit antibody
  • Documents
  • Description
    phospho-AKT1 (Thr450) Rabbit monoclonal antibody
  • Tested applications
    WB, IHC-P, IP
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic phosphopeptide corresponding to residues surrounding Thr450 of human AKT1
  • Clonality
    Monoclonal
  • Formulation
    50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    IHC: 1/20

    IP: 1/20

  • Validations

    Western blot detection of phospho-AKT1 (Thr450) in U2OS,C6 cell lysates using phospho-AKT1(Thr450) Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.

    Western blot detection of phospho-AKT1 (Thr450) in U2OS,C6 cell lysates using phospho-AKT1(Thr450) Rabbit mAb(1:1000 diluted).Predicted band size:56kDa.Observed band size:56kDa.

  • Background
    The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"