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Product Name
Anti-AKT1 Rabbit antibody
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Description
AKT1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human AKT1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.
Western blot analysis of AKT1 expression in C2C12,THP-1,3T3 and Jurkat cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.
Western blot analysis of AKT1 expression in MCF-7 cell lysate.
Western blot analysis of AKT1 expression in His-AKT1 trans CHO-K1,His-AKT2 trans CHO-K1 and CHO-K1 cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.
Immunofluorescent analysis of Hela cells, using AKT1 Antibody.
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Background
Swiss-Prot Acc.P31749.Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"