Anti-AKT1 Rabbit antibody

Cat.#: 166709

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Product Information

  • Product Name
    Anti-AKT1 Rabbit antibody
  • Documents
  • Description
    AKT1 Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP, FC
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthesized peptide derived from human AKT1
  • Clonality
    Polyclonal
  • Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500-1/2000

    IHC: 1/50-1/200

    ICC/IF: 1/50-1/200

    IP: 1/50

  • Validations

    Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.

    Immunohistochemical analysis of paraffin-embedded human colon, using AKT1 Antibody.

    Western blot analysis of AKT1 expression in C2C12,THP-1,3T3 and Jurkat cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.

    Western blot analysis of AKT1 expression in C2C12,THP-1,3T3 and Jurkat cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.

    Western blot analysis of AKT1 expression in MCF-7 cell lysate.

    Western blot analysis of AKT1 expression in MCF-7 cell lysate.

    Western blot analysis of AKT1 expression in His-AKT1 trans CHO-K1,His-AKT2 trans CHO-K1 and CHO-K1 cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.

    Western blot analysis of AKT1 expression in His-AKT1 trans CHO-K1,His-AKT2 trans CHO-K1 and CHO-K1 cell lysates using AKT1 antibody at 1/1000 dilution.Predicted band size:60KDa.Observed band size:60KDa.

    Immunofluorescent analysis of Hela cells, using AKT1 Antibody.

    Immunofluorescent analysis of Hela cells, using AKT1 Antibody.

  • Background
    Swiss-Prot Acc.P31749.Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"