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Product Name
Anti-Cytokeratin 18 Rabbit antibody
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Description
Cytokeratin 18 Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
K18; CK-18; CYK18 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Cytokeratin 18
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
1:2000-1:10000
1:20-1:50
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Validations
Western blot detection of Cytokeratin 18 in K562,Hela cell lysates using Cytokeratin 18 Rabbit mAb(1:1000 diluted).Predicted band size:48kDa.Observed band size:48kDa.
Immunocytochemistry of Cytokeratin 18(green) in Hela cells using Cytokeratin 18 Rabbit mAb at dilution 1/200, and DAPI(blue)
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Background
Swiss-Prot Acc.P05783.Involved in the uptake of thrombin-antithrombin complexes by hepatic cells . When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"