-
Product Name
Anti-Cytokeratin 18 (4G8) Mouse antibody
- Documents
-
Description
Cytokeratin 18 (4G8) Mouse monoclonal antibody
-
Tested applications
WB, ICC/IF
-
Species reactivity
Human, Mouse, Rat
-
Isotype
IgG1
-
Preparation
Antigen: Recombinant Protein of Keratin type I cytoskeletal 18
-
Clonality
Monoclonal
-
Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/1000 -1/3000
IF: 1/100-1/200
-
Validations
Western blot detection of Keratin 18 in 3T3,Hela cell lysates using Keratin 18 (4G8) Mouse mAb(1:1000 diluted).Predicted band size:46KDa.Observed band size:46KDa.
IF analysis of Hela, and DAPI (Right) diluted at 1:100.
Western blot analysis of 1) HepG2, 2) Hela, 3) Mouse Liver tissue, 4) C2C12, 5) Rat Heart tissue, 6) Mouse Skeletal Muscle tissue, diluted at 1:2000.
-
Background
Swiss-Prot Acc.P05783.Involved in the uptake of thrombin-antithrombin complexes by hepatic cells . When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"