-
Product Name
Cytokeratin 18 antibody
- Documents
-
Description
Cytokeratin 18 Mouse Monoclonal antibody. Positive IF detected in HeLa cells, HepG2 cells. Positive IHC detected in human kidney tissue, human breast cancer tissue, human lung cancer tissue. Positive FC detected in MCF-7 cells. Positive WB detected in HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 48 kDa
-
Tested applications
ELISA, FC, IF, IHC, WB
-
Species reactivity
Human; other species not tested.
-
Alternative names
CK 18 antibody; CYK18 antibody; Cytokeratin 18 antibody; K18 antibody; keratin 18 antibody; KRT18 antibody
- Immunogen
-
Isotype
Mouse IgG1
-
Preparation
This antibody was obtained by immunization of Cytokeratin 18 recombinant protein (Accession Number: NM_000224). Purification method: Protein G purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:100-1:1000
IF: 1:20-1:200
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107231(KRT18 Antibody) at dilution of 1:20000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107231(KRT18 Antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:107231(KRT18 Antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0
Immunofluorescent analysis of HeLa cells using Catalog No:107231(KRT18 Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
1X10^6 MCF-7 cells were stained with 0.2ug KRT18 antibody (Catalog No:107231, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.
-
Background
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"