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Product Name
Anti-Cytokeratin 18 (6A8) Mouse antibody
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Description
Cytokeratin 18 (6A8) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant fragment of human Cytokeratin 18 (aa391-483) expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), hepatocarcinoma (B), stomach cancer (C) and colon cancer tissue (D), showing cytoplasmic location with DAB staining using CK18 mouse mAb.
Western blot detection of Cytokeratin 18 in A549 and Hela cell lysates using Cytokeratin 18 mouse mAb (1:1000 diluted).Predicted band size:48KDa.Observed band size:48KDa.
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Background
Swiss-Prot Acc.P05783.Cytokeratin 18, also known as CK18, CYK18, KRT18. Entrez Protein NP_000215. It encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"