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Proper pH of fixative and good mounting media is the essential for a good signal to noise ratio. You may want to make up fresh 4% (or less) PFA, which can be stored at 4 degrees Celsius for a week or so.For a 4% PFA solution follow this protocol.Add ...

Add 1/10 vol of NH4OAc, en 2.5 volume 100% cold EtOH (100%) and 1 µl glycogen (if you think you'll the pellet without see glycogen you can do without it)mixIncubate 30 min at -80°CCentrifuge 20 min at 12000g at 4°C(remove supernatant)Wash with 75...

The pH dependence is usually due to the side groups of the amino acids. A change in pH changes the protonation pattern and can, in extreme cases, result in protein denaturation. I therefore suspect that your protein stabilizes the enzyme structure, t...

Introduction: Empathy is also known as sympathy, etc. It refers to the ability to sense other people's feelings. It has been interpreted in the field of psychology. But recently, scientists at the University of Cambridge in the United Kingdom hav...

We do data normalization when seeking for relations. Some people do this methods, unfortunately, in experimental designs, which is not correct except if the variable is a transformed one, and all the data needs the same normalization method, such as ...

Tissue cells are inherently suicidal and they require growth factors that not only stimulate replication but are generally required to tell the cell not to undergo apoptosis. Remember that most cells (differentiated or not) know were they are because...

There are several differences. But first, you have to differentiate between different experiments with different objectives in different species.a) Mice are not man: Standard anti-mouse anti-CD3 antibody (hamster-anti-mouse 145-2C11) has intermediate...

There are some articles on this matter:= Formaldehyde (Formalin) fixation at lower temperature (4°C) better preserves Nucleic acid integrity (2011), cf.( http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021043)= Formaldehyde (Forma...

Introduction: For a long time, scientists believe that every day, the human brain's hippocampus will produce hundreds of new nerve cells. However, one of the latest results published in Nature may have to subvert this idea. The new study found th...

SDS-PAGE points to consider.1. NEVER take an SDS-PAGE for face-value! People do this all to often. If you see one 50kDa band, and nothing else, great, you've got protein >95% pure. If you see multiple bands, you may have your protein really pu...