Rat UCHL3 (His Tag) recombinant protein

Rat UCHL3 (His Tag) recombinant protein

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Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3, and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains". Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome (By similarity).

Ubiquitin carboxyl-terminal hydrolase isozyme L3

UCHL3; UCH-L3; RP11-173B14.3

Q91Y78

Uchl3

E. coli

A DNA sequence encoding the rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was fused with a polyhistidine tag at the N-terminus.

Rat

The recombinant rat UCHL3 comprises 240 amino acids and predicts a molecular mass of 27.5 kDa. The apparent molecular mass of the rat UCHL3 is approximately 28 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/μg.