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Product Name
Anti-UCHL3 antibody
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Description
Rabbit monoclonal to UCHL3
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Tested applications
ELISA, WB, IP
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Species reactivity
Rat UCHL3
Has cross-reactivity in ELISA with Human UCHL3; Mouse UCHL3 -
Alternative names
RP11-173B14.3 antibody; UCHL3 antibody; Uchl3 antibody; UCH-L3 antibody; UCH-L3 antibody; UCH-L3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Rat UCHL3 (rR UCHL3; Q91Y78; Glu2-Ala230).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat UCHL3. The detection limit for Rat UCHL3 is approximately 0.0049 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
UCHL3 Antibody, Rabbit MAb, Western blot
UCHL3 Antibody, Rabbit MAb, Immunoprecipitation
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Background
Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
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References
- Wood,M.A. et al., 2005, Hippocampus 15 (5):610-21.
- Kwon,J. et al., 2006, Exp Anim 55 (1):35-43.
- Setsuie,R. et al., 2009, Neurochem Int 54 (5-6):314-21.
- Setsuie,R. et al., 2010, Neurochem Int 56 (8):911-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"