Human UCHL3 (His Tag) recombinant protein

Human UCHL3 (His Tag) recombinant protein

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Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3", and exhibits a preference towards 'Lys-48'-linked Ubiquitin chains. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. Required for stress-response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders.

Ubiquitin carboxyl-terminal hydrolase isozyme L3

UCHL3; UCH-L3; RP11-173B14.3

P15374

UCHL3

E. coli

A DNA sequence encoding the human UCHL3 (NP_005993.1) (Glu 2-Ala 230) was expressed, with a polyhistide tag at the N-terminus.

Human

The recombinant human UCHL3 consisting of 236 amino acids and has a calculated molecular mass of 27 kDa. It migrates as an approximately 26 kDa band in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/μg.