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Product Name
UCHL3 antibody
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Description
UCHL3 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells, rat spleen tissue. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Ubiquitin carboxyl terminal hydrolase isozyme L3 antibody; Ubiquitin thioesterase L3 antibody; UCHL3 antibody; UCH-L3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of UCHL3 recombinant protein (Accession Number: NM_006002). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116672(UCHL3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116672(UCHL3 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-UCHL3 (IP:Catalog No:116672, 4ug; Detection:Catalog No:116672 1:500) with HeLa cells lysate 1600ug.
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Background
UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"