UCHL3 antibody

Cat.#: 116672

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Product Information

  • Product Name
    UCHL3 antibody
  • Documents
  • Description
    UCHL3 Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells, rat spleen tissue. Observed molecular weight by Western-blot: 26 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Ubiquitin carboxyl terminal hydrolase isozyme L3 antibody; Ubiquitin thioesterase L3 antibody; UCHL3 antibody; UCH-L3 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of UCHL3 recombinant protein (Accession Number: NM_006002). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116672(UCHL3 antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116672(UCHL3 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116672(UCHL3 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:116672(UCHL3 antibody) at dilution of 1:50 (under 10x lens)

    IP Result of anti-UCHL3 (IP:Catalog No:116672, 4ug; Detection:Catalog No:116672 1:500) with HeLa cells lysate 1600ug.

    IP Result of anti-UCHL3 (IP:Catalog No:116672, 4ug; Detection:Catalog No:116672 1:500) with HeLa cells lysate 1600ug.

  • Background
    UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"