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Product Name
Mouse Coagulation Factor III / Tissue Factor / CD142 (His Tag) recombinant protein
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Description
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
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Protein name
Tissue factor
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Protein short names
TF; COAGULATION FACTOR III; TISSUE FACTOR; CF-3; CF3; F3; AA409063; CD142; TFA; THROMBOPLASTIN
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Uniprot ID
P20352
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Gene Name
F3; Cf-3; Cf3
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse F3 (NP_034301.3) (Met 1-Glu 251) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Mouse
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Molecular weight
The secreted recombinant mouse F3 consists of 234 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC (R&D Systems, Catalog# ES011). The AC50 is < 5 μg/ml.
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Validations
Mouse Coagulation Factor III / Tissue Factor / CD142 Protein (His Tag) SDS-PAGE
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