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Product Name
Human SAE1 (His & GST Tag) recombinant protein
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Description
SAE1 belongs to the ubiquitin-activating E1 family. It is a heterodimer that acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It functions as a UBLI E1 ligase mediating the ATP-dependent activation of UBL1. SAE1 binds with UBLE1A and UBLE1B to form a heterodimer which can bind UBL1. SAE1 also regulates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins.
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Protein name
SUMO-activating enzyme subunit 1
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Protein short names
UBLE1A; UBIQUITIN-LIKE (SENTRIN) ACTIVATING ENZYME E1A; SUMO-ACTIVATING ENZYME SUBUNIT 1; 2400010M20RIK; AW743391; HSPC140; UBIQUITIN-LIKE 1 (SENTRIN) ACTIVATING ENZYME E1A; SUMO-1 ACTIVATING ENZYME E1 N SUBUNIT; UBIQUITIN-LIKE 1-ACTIVATING ENZYME E1A; D7ERTD177E; SENTRIN/SUMO-ACTIVATING PROTEIN AOS1; UBIQUITIN-LIKE PROTEIN SUMO-1 ACTIVATING ENZYME; ACTIVATOR OF SUMO1; SUA1; 2610044L12RIK; AOS1; UBIQUITIN-LIKE 1 (SENTRIN) ACTIVATING ENZYME SUBUNIT 1; SUMO-1 ACTIVATING ENZYME SUBUNIT 1; AL033372
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Uniprot ID
Q9UBE0
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Gene Name
SAE1; AOS1; SUA1; UBLE1A
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human SAE1 (Q9UBE0) (Met1-Lys346) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human SAE1 /GST chimera consists of 583 amino acids and has a calculated molecular mass of 66.3 kDa. The recombinant protein migrates as an approximately 61 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human SAE1 Protein (His & GST Tag) SDS-PAGE
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