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Product Name
SAE1 antibody
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Description
SAE1 Rabbit Polyclonal antibody. Positive WB detected in A431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue, human skin cancer tissue. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AOS1 antibody; FLJ3091 antibody; HSPC140 antibody; SAE1 antibody; SUA1 antibody; UBLE1A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of SAE1 recombinant protein (Accession Number: NM_005500). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:115042(SAE1 antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:115042(SAE1 Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:115042(SAE1 Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of HeLa cells using Catalog No:115042(SAE1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).
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References
- Chen D, Zheng W, Lin A, Uyhazi K, Zhao H, Lin H. Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis. Current biology : CB. 22(5):420-5. 2012.
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