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Product Name
Anti-SAE1 Rabbit antibody
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Description
SAE1 Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human, Rat
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Alternative names
AOS1; SUA1; UBLE1A; HSPC140 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human SAE1
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
1:2000-1:10000
1:100
1:50
1:20
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Validations
Western blot detection of SAE1 in K562,Hela cell lysates using SAE1 Rabbit mAb(1:1000 diluted).Predicted band size:39kDa.Observed band size:39kDa.
Immunocytochemistry of SAE1(green) in Hela cells using SAE1 Rabbit mAb at dilution 1/200, and DAPI(blue)
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Background
Swiss-Prot Acc.Q9UBE0.The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"