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STAT3 antibody

Cat.#: 115688

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Product Information

  • Product Name
    STAT3 antibody
  • Documents
  • Description
    STAT3 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, A549 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse brain tissue, mouse heart tissue, mouse lung tissue, mouse thymus tissue, NIH/3T3 cells, rat brain tissue. Positive IP detected in HeLa cells. Positive FC detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human breast cancer tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 88 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Acute phase response factor antibody; APRF antibody; FLJ20882 antibody; HIES antibody; STAT3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of STAT3 recombinant protein (Accession Number: XM_017024975). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IP: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115688(STAT3 antibody) at dilution of 1:4000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:115688(STAT3 antibody) at dilution of 1:4000

    Immunohistochemistry of paraffin-embedded human cervical cancer slide using Catalog No:115688(STAT3 Antibody) at dilution of 1:50

    Immunohistochemistry of paraffin-embedded human cervical cancer slide using Catalog No:115688(STAT3 Antibody) at dilution of 1:50

    1X10^6 HeLa cells were stained with 0.2ug STAT3 antibody (Catalog No:115688, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HeLa cells were stained with 0.2ug STAT3 antibody (Catalog No:115688, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    IP Result of anti-STAT3 (IP:Catalog No:115688, 3ug; Detection:Catalog No:115688 1:2000) with HeLa cells lysate 3000ug.

    IP Result of anti-STAT3 (IP:Catalog No:115688, 3ug; Detection:Catalog No:115688 1:2000) with HeLa cells lysate 3000ug.

    Immunofluorescent analysis of HeLa cells using Catalog No:115688(STAT3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

    Immunofluorescent analysis of HeLa cells using Catalog No:115688(STAT3 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

  • Background
    Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3.
  • References
    • Cai Y, Chen H, Jin L, You Y, Shen J. STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage. Parasites & vectors. 6:356. 2013.
    • Cai Y, Chen H, Mo X. Toxoplasma gondii inhibits apoptosis via a novel STAT3-miR-17-92-Bim pathway in macrophages. Cellular signalling. 26(6):1204-12. 2014.
    • Xiang M, Su H, Hong Z, Yang T, Shu G. Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 80:62-71. 2015.
    • Xing WJ, Liao XH, Wang N. MRTF-A and STAT3 promote MDA-MB-231 cell migration via hypermethylating BRSM1. IUBMB life. 67(3):202-17. 2015.
    • Dong Z, Lu X, Yang Y. IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation. BMC pulmonary medicine. 15:13. 2015.
    • Rathinavelu A, Narasimhan M, Muthumani P. A novel regulation of VEGF expression by HIF-1α and STAT3 in HDM2 transfected prostate cancer cells. Journal of cellular and molecular medicine. 16(8):1750-7. 2012.
    • Ho KY, Huang HH, Hung KF. Cholesteatoma growth and proliferation: relevance with serpin B3. The Laryngoscope. 122(12):2818-23. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"