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Anti-STAT3 (5G6) Mouse antibody

Cat.#: 168434

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-STAT3 (5G6) Mouse antibody
  • Documents
  • Description
    STAT3 (5G6) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human STAT3 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IHC: 1/200 - 1/1000

    ICC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Immunofluorescence analysis of Hela cells using STAT3 mouse mAb (green). Blue

    Immunofluorescence analysis of Hela cells using STAT3 mouse mAb (green). Blue

    Western blot detection of Stat3 in Hela cell lysates using Stat3 mouse mAb (1:1000 diluted).Predicted band size:88KDa.Observed band size:88KDa.

    Western blot detection of Stat3 in Hela cell lysates using Stat3 mouse mAb (1:1000 diluted).Predicted band size:88KDa.Observed band size:88KDa.

    Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.

    Immunohistochemical analysis of paraffin-embedded mammary cancer tissues (left) and lung cancer tissues (right) using STAT3 mouse mAb with DAB staining.

  • Background
    Swiss-Prot Acc.P40763.The Stat3 transcription factor is an important signaling molecule for many cytokines and growth-factor receptors and is required for murine fetal development . Stat3 is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding. Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways. Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure or cell maturation stage. It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain.Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"