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Product Name
Human Semaphorin 4D/SEMA4D/CD100 (His Tag) recombinant protein
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Description
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
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Protein name
Semaphorin-4D
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Protein short names
MGC169141; XXYAC-YM68G7.1; C9ORF164; FLJ46484; FLJ33485; SEMAPHORIN 4D; M-SEMA-G; SEMACL2; SEMA4D; COLL-4; FLJ39737; SEMAJ; SEMCL2; FLJ34282; MGC169138; CD100
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Uniprot ID
Q92854
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Gene Name
SEMA4D; C9orf164; CD100; SEMAJ
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human SEMA4D isoform 1 (Q92854-1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human SEMA4D consists of 725 amino acids and predictes a molecular mass of 80.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSEMA4D is approximately 110 kDa due to glycosylation.
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Purity
> 92 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc (Cat:501204) with a linear range of 0.625-5 μg/ml. -
Validations
Human Semaphorin 4D / SEMA4D / CD100 Protein (His Tag) SDS-PAGE
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