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Product Name
Anti-Semaphorin 4D/SEMA4D/CD100 antibody
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Description
Mouse monoclonal to Semaphorin 4D/SEMA4D/CD100
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Tested applications
FCM
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Species reactivity
Human Semaphorin 4D / SEMA4D / CD100
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Alternative names
C9orf164 antibody; CD100 antibody; CD100 antibody; coll-4 antibody; coll-4 antibody; FLJ33485 antibody; FLJ34282 antibody; FLJ39737 antibody; M-sema-G antibody; SEMA4D antibody; Semacl2 antibody; SEMAJ antibody; Semaphorin 4D antibody; FLJ46484 antibody; MGC169138 antibody; MGC169141 antibody; CD100 antibody; SEMAJ antibody; coll-4 antibody; C9orf164 antibody; M-sema-G antibody; CD100 antibody; Semaj antibody; Semcl2 antibody; coll-4 antibody; Semacl2 antibody; Sema4d antibody; Semaj antibody; Semaphorin 4D antibody; Semcl2 antibody; XXyac-YM68G7.1 antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Semaphorin 4D / SEMA4D / CD100 (rh Semaphorin 4D / SEMA4D / CD100; Q92854-1; Met1-Arg734). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
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Validations
Semaphorin 4D / SEMA4D / CD100 Antibody, Mouse MAb, Flow Cytometry
Flow cytometric analysis of Human SEMA4D(CD100) expression on human whole blood lymphocytes. Cells were stained with purified anti-Human SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.
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Background
Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"