Rhesus CD16 / FCGR3 (His & AVI Tag) recombinant protein

Rhesus CD16 / FCGR3 (His & AVI Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

FCR-10; FCGAMMARIV; FCRIII; FCGRIV; CD16; FCRIIIA; CD16-2; FCG3; FC GAMMA RIII; IGFR3; FCGRIII; RIII; FCGR4; FCGR3; FC Γ FCRIIIB; FCGR3A

F5A869

Human Cells

A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Rhesus

The recombinant rhesus CD16 consists of 223 amino acids and has a calculated molecular mass of 25.3 kDa.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Rhesus CD16-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC₅₀ of biotinylated human IgG1 is 0.2-0.5 μg/ml.