Rhesus CD16 / FCGR3 (His Tag) recombinant protein

Rhesus CD16 / FCGR3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

FCGAMMARIV; FCR-10; FCGRIV; FCRIII; FCRIIIA; CD16; FC GAMMA RIII; FCG3; CD16-2; FCGR4; RIII; IGFR3; FCGRIII; FCGR3A; FCRIIIB; FC Γ FCGR3

F5A869

Human Cells

A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1-Gly 206) was fused with a polyhistidine tag at the C-terminus.

Rhesus

The recombinant rhesus CD16 consists of 201 amino acids and has a calculated molecular mass of 22.7 kDa.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Rhesus FCGR3-His (cat:504599) at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) (Cat:500583). The EC₅₀ of human IgG1 (Fc) (Cat:500583) is 0.35-0.81 μg/ml.