-
Product Name
Anti-CD16 / FCGR3 antibody
- Documents
-
Description
Rabbit polyclonal to CD16 / FCGR3
-
Tested applications
ELISA, IHC-P
-
Species reactivity
Cynomolgus CD16 / FCGR3
-
Alternative names
CD16-2 antibody; CD16 antibody; CD16 antibody; Fc &gamma antibody; Fc gamma RIII antibody; FCG3 antibody; FcgammaRIV antibody; FCGR3 antibody; Fcgr3 antibody; Fcgr3a antibody; FCGR3A antibody; Fcgr4 antibody; FCGRIII antibody; FcgRIV antibody; FCR-10 antibody; FCRIII antibody; FcRIII antibody; FCRIIIA antibody; FCRIIIB antibody; IGFR3 antibody; RIII antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus CD16 / FCGR3 (rC CD16 / FCGR3; ABD83657.1; Met 1-Gly 206). CD16 / FCGR3 specific IgG was purified by Cynomolgus CD16 / FCGR3 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus FCGR3. The detection limit for Cynomolgus FCGR3 is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
-
Validations
CD16 / FCGR3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of cynomolgus macaque FCGR3 in cynomolgus macaque lung with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
CD16 / FCGR3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of cynomolgus macaque FCGR3 in cynomolgus macaque spleen with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
-
References
- Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
- Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
- Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"