Rhesus CD16 / FCGR3 (His & AVI Tag) recombinant protein

Rhesus CD16 / FCGR3 (His & AVI Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

FCR-10; FCGAMMARIV; CD16; FCRIIIA; FCRIII; FCGRIV; CD16-2; FCG3; FC GAMMA RIII; FCGR3; FC Γ FCRIIIB; FCGR3A; IGFR3; FCGRIII; RIII; FCGR4

F5A869

Human Cells

A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.

Rhesus

The recombinant rhesus CD16 consists of 223 amino acids and has a calculated molecular mass of 25.3 kDa.

> 92 % as determined by SDS-PAGE

1. Measured by its ability to bind human IgG1 in a functional ELISA.
2. Labeling ratio of biotin to protein: 0.4