Cynomolgus c-Met/HGFR (Fc Tag) recombinant protein

Cynomolgus c-Met/HGFR (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Receptor protein-tyrosine kinase

F7H1K4

MET; EGK_14061

Human Cells

A DNA sequence encoding the cynomolgus/rhesus MET (NP_001162100.1) (Met1-Thr932) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus MET sequences are identical.

Cynomolgus

The recombinant cynomolgus/rhesus MET comprises 1146 amino acids and has a calculated molecular mass of 128.3 KDa. The apparent molecular mass of it is approximately 100.2 and 42.5 KDa in SDS-PAGE under reducing conditions.

(66.7+31.5) % as determined by SDS-PAGE

Immobilized Cynomolgus HGF (Cat:503008) at 10 μg/ml (100 μl/well) can bind Cynomolgus MET-Fc, EC₅₀ of Cynomolgus MET-Fc is 0.04-0.09 μg/ml.