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Product Name
Human c-Met/HGFR (His & Fc Tag) recombinant protein
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Protein short names
MET; HGFR; PAR4; AI838057; RCCP2; AUTS9; C-MET; HGF
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Uniprot ID
P08581
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Thr 932) of human c-Met (NP_000236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Human
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Molecular weight
The mature recombinant human c-Met/Fc is a disulfide-linked tetramer composed of two proteolytically cleaved α and β subunits. Each α and β together with the C-terminal Fc tag consists of 1155 amino acids and has a predicted molecular mass of 129.5 (α =32.5 + Fc tagged β=97) kDa. The rh c-MET/Fc heterodimer thus migrates with apparent molecular mass of approximately 57.8 kDa and 130 kDa respectively in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.Immobilized recombinant human HGF at 1 μg/ml (100ul/well) can bind Human c-MET / HGFR with a linear range of 0.31-160ng/ml. Scatchard analysis showed the affinity constant (Kd) of human HGF bound to human c-MET / HGFR was 0.52 nM.
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Validations
Human c-MET / HGFR Protein (His & Fc Tag) SDS-PAGE
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