Canine c-Met/HGFR (His Tag) recombinant protein

Canine c-Met/HGFR (His Tag) recombinant protein

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Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells (By similarity).

Hepatocyte growth factor receptor

C-MET; AUTS9; RCCP2; HGF; HGFR; MET; AI838057; PAR4

Q75ZY9

MET

Human Cells

A DNA sequence encoding the extracellular domain of canine MET (NP_001002963.1) (Met 1-Leu 935) was expressed, with a polyhistidine tag at the N-terminus.

Canine

The recombinant canine c-Met is a disulfide-linked heterodimer composed of proteolytically cleaved ? and ? subunits. Each ? and ? subunit together consists of 922 amino acids and has a predicted molecular mass of 103 (? =33 + ? =70) kDa. As a result of glycosylation, the apparent molecular mass of the canine c-Met is approximately 42-47 kDa and 85-95 kDa respectively in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human HGF (Cat:502454) at 10 μg/mL (100 μl/well) can bind canine c-MET (Cat:500965), The EC₅₀ of canine c-MET (Cat:500965) is 7 ng/mL.
3. Immobilized canine MET-His at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-His (Cat:10463-H08H), The EC₅₀ of biotinylated human HGF-His (Cat:10463-H08H) is 0.11-0.25 μg/ml.