Anti-Met antibody

Cat.#: 175797

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Product Information

  • Product Name
    Anti-Met antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Met
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    P antibody; HGF antibody; HGFR antibody; Par4 antibody; c-Met antibody; AI838057 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within mouse cmet aa 650-690.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000

    ICC: 1:200

    IHC-P: 1:200

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of cMet on different cell lysates using anti-cMet antibody at 1/1000 dilution.; Positive control:; Lane 1: Mouse liver; Lane 2: Mouse kidney; Lane 3: D3; Lane 4: MEF

    Fig1: Western blot analysis of cMet on different cell lysates using anti-cMet antibody at 1/1000 dilution.; Positive control:; Lane 1: Mouse liver; Lane 2: Mouse kidney; Lane 3: D3; Lane 4: MEF

    Fig2: ICC staining cMet in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining cMet in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining cMet in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: ICC staining cMet in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining cMet in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining cMet in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-cMet antibody. Counter stained with hematoxylin.

    Fig8: Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

    Fig8: Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

  • Background
  • References
    • Essential role for the c-met receptor in the migration of myogenic precursor cells into the limb bud. Bladt F., Riethmacher D., Isenmann S., Aguzzi A., Birchmeier C. Nature 376:768-771(1995)
    • MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met. Higuchi T., Orita T., Katsuya K., Yamasaki Y., Akiyama K., Li H., Yamamoto T., Saito Y., Nakamura M. Mol. Cell. Biol. 24:7456-7468(2004)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"