Mouse PARP-1/PARP (His Tag) recombinant protein

Mouse PARP-1/PARP (His Tag) recombinant protein

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Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Poly (ADP-ribose) polymerase family, member 1

SPARP-1; PARP-1; C80510; PPOL; PARP; RP11-125A15.2; ADPRT1; ADPRT; PADPRT-1; ARTD1; ADPRP; AI893648; PARP1; 5830444G22RIK

Q921K2

Parp1; Adprt1; mCG_19846

Baculovirus-Insect Cells

A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.

Mouse

The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.

> 85 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind  biotinylated human HSP70, The EC₅₀ of biotinylated human HSP70 is 0.021 μg/mL.