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Anti-PARP1 (10C2) Mouse antibody

Cat.#: 168189

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Product Information

  • Product Name
    Anti-PARP1 (10C2) Mouse antibody
  • Documents
  • Description
    PARP1 (10C2) Mouse monoclonal antibody
  • Tested applications
    ICC/IF, WB, IHC-P
  • Species reactivity
    Human
  • Isotype
    IgG1
  • Preparation
    Antigen: Synthetic Peptide of Cleaved PARP
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    IF: 1/50-200

    WB: 1/2000-5000

    IHC: 1/50-300

  • Validations

    Immunohistochemical analysis of paraffin-embedded human Tonsil Tissue using Cleaved PARP uff08uff09Mouse mAb diluted at 1:500.

    Immunohistochemical analysis of paraffin-embedded human Tonsil Tissue using Cleaved PARP uff08uff09Mouse mAb diluted at 1:500.

    Western blot analysis of extracts from Hela,MCF7 and Jurkat cell lysates using PARP (10C2) Mouse mAb (1:2000 diluted).Predicted band size:116, 89KDa.Observed band size:116KDa.

    Western blot analysis of extracts from Hela,MCF7 and Jurkat cell lysates using PARP (10C2) Mouse mAb (1:2000 diluted).Predicted band size:116, 89KDa.Observed band size:116KDa.

    Western blot detection of PARP in K562,Ramos,Jurkat cell lysates using PARP (10C2) Mouse mAb(1:1000 diluted).Predicted band size:116KDa.Observed band size:116/89KDa.

    Western blot detection of PARP in K562,Ramos,Jurkat cell lysates using PARP (10C2) Mouse mAb(1:1000 diluted).Predicted band size:116KDa.Observed band size:116/89KDa.

  • Background
    Swiss-Prot Acc.P09874.Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
  • References
    • MUC1 activates JNK1 and inhibits apoptosis under genotoxic stress

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"