Human PARP-1/PARP (His Tag) recombinant protein

Human PARP-1/PARP (His Tag) recombinant protein

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Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Poly (ADP-ribose) polymerase family, member 1, isoform CRA_a

PPOL; C80510; PARP-1; SPARP-1; PARP; PADPRT-1; ADPRT; ARTD1; ADPRT1; RP11-125A15.2; AI893648; ADPRP; 5830444G22RIK; PARP1

P09874

PARP1; hCG_14746

Baculovirus-Insect Cells

The amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human PARP1 consists of 1024 amino acids and predicts a molecular mass of 114.5 kDa. The apparent molecular mass of rhPARP1 is approximately 100-110 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human PARP1 at 10 μg/mL (100 μl/well) can bind  biotinylated human HSP70, The EC₅₀ of biotinylated human HSP70 is 0.035 μg/mL.