-
Product Name
Anti-PARP1 Rabbit antibody
- Documents
-
Description
PARP1 Rabbit polyclonal antibody
-
Tested applications
WB, IHC-P, ICC/IF, FC
-
Species reactivity
Human, Mouse, Rat
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthesized peptide derived from human PARP
-
Clonality
Polyclonal
-
Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/500-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
FC: 1/50
-
Validations
Western blot analysis of PARP expression in Jurkat and Hela cell lysates using PARP antibody at 1/1000 dilution.Predicted band size:113KDa.Observed band size:113KDa.
Western blot analysis of PARP expression in Jurkat cell lysate.
Immunohistochemical analysis of paraffin-embedded human testis, using PARP Antibody.
Immunofluorescent analysis of Hela cells, using PARP Antibody .
-
Background
Swiss-Prot Acc.P09874.Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
-
References
- LIPUS promotes FOXO1 accumulation by downregulating miR-182 to enhance osteogenic differentiation in hPDLCs
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"