Anti-PARP1 Rabbit antibody

Cat.#: 166851

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Product Information

  • Product Name
    Anti-PARP1 Rabbit antibody
  • Documents
  • Description
    PARP1 Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human PARP1
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000

    IHC: 1/200

    ICC/IF: 1/50

  • Validations

    Western blot detection of PARP1 in K562,3T3,Hela cell lysates using PARP1 Rabbit pAb(1:1000 diluted).Predicted band size:113kDa.Observed band size:116/89kDa.

    Western blot detection of PARP1 in K562,3T3,Hela cell lysates using PARP1 Rabbit pAb(1:1000 diluted).Predicted band size:113kDa.Observed band size:116/89kDa.

    Immunocytochemistry of PARP1(green) in Hela cells using PARP1 Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of PARP1(green) in Hela cells using PARP1 Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunohistochemistry of PARP1 in paraffin-embedded Human tonsil using PARP1 Rabbit pAb at dilution 1/50

    Immunohistochemistry of PARP1 in paraffin-embedded Human tonsil using PARP1 Rabbit pAb at dilution 1/50

  • Background
    Swiss-Prot Acc.P09874.Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:17177976, PubMed:18172500, PubMed:19344625, PubMed:19661379, PubMed:23230272). Mediates the poly(ADP-ribosyl)ation of APLF and CHFR (PubMed:17396150). Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production (PubMed:17177976). Required for PARP9 and DTX3L recruitment to DNA damage sites (PubMed:23230272). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272). Mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). Mediates the poly(ADP-ribosyl)ation of histones in a HPF1-dependent manner (PubMed:27067600). Involved in the synthesis of ATP in the nucleus, together with NMNAT1, PARG and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"