Mouse KIT / c-KIT / CD117 (His Tag) recombinant protein

Mouse KIT / c-KIT / CD117 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

Mast/stem cell growth factor receptor Kit

GSFSCO1; SOW3; GSFSCO5; TR-KIT; PBT; SCFR; W; CD117; SSM; GSFSOW3; FDC; C-KIT; SCO1; BS; SCO5

P05532

Kit; Sl

Human Cells

A DNA sequence encoding the mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse KIT comprises 510 amino acids and has a predicted molecular mass of 57 kDa. As a result of glycosylation, the apparent molecular mass of rmKIT is approximately 70-80 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse KIT at 2 μg/ml (100 μl/well) can bind biotinylated mouse KITL with a linear ranger of 1.28-6.4 ng/ml.