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Product Name
Anti-c-Kit (7F12) Mouse antibody
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Description
c-Kit (7F12) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human KIT expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IHC: 1/200 - 1/1000
ELISA: 1/10000
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Validations
Western blot analysis of extracts from Jurkat, A549 and MCF7 cell lysates using c-Kit mouse mAb (1:1000 diluted).Predicted band size:145KDa.Observed band size:120~145KDa.
Western blot analysis using KIT mouse mAb against Jurkat (1) and Hela (2) cell lysate.
Immunohistochemical analysis of paraffin-embedded gastrointestinal stromal tumor using KIT mouse mAb with DAB staining.
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Background
Swiss-Prot Acc.P10721.The c-Kit proto-oncogene is a member of the receptor tyrosine kinase family and, more specifically, is closely related to the platelet derived growth factor receptor (PDGFR). c-Kit, the normal cellular homolog of the HZ4-feline sarcoma virus transforming gene (v-Kit), encodes a transmembrane receptor. c-Kit regulates a variety of biological responses including chemotaxis, cell prolif- eration, apoptosis and adhesion. c-Kit is also identical with the product of the W locus in mice and, as such, is integral to the development of mast cells and hematopoiesis. The ligand for the c-Kit receptor (KL) has been identified and is encoded at the murine steel (SI) locus. Kit is the human homolog of the proto- oncogene c-Kit. Mutations in Kit are integral for tumor growth and progression in various cancers.
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