Mouse KIT / c-KIT / CD117 (Fc Tag) recombinant protein

Mouse KIT / c-KIT / CD117 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Mast/stem cell growth factor receptor Kit

SCFR; PBT; GSFSCO1; GSFSCO5; SOW3; TR-KIT; GSFSOW3; SCO1; C-KIT; FDC; SCO5; BS; W; CD117; SSM

P05532

Kit; Sl

Human Cells

A DNA sequence encoding the mouse KIT isoform 1 (NP_001116205.1) extracellular domain (Met 1-Thr 523) was fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse KIT/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 740 amino acids and has a predicted molecular mass of 82.5 kDa. As a result of glycosylation, the apparent molecular mass of rmKIT/Fc monomer is approximately 100-110 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 μg/ml (100 μl/well) can bind mouse KIT / CD117 with a linear ranger of 1.28-32 ng/ml.