Human KIT / c-KIT / CD117 (Fc Tag) recombinant protein

Human KIT / c-KIT / CD117 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

Mast/stem cell growth factor receptor Kit

TR-KIT; GSFSCO5; SOW3; GSFSCO1; SCFR; PBT; SSM; CD117; W; SCO5; BS; C-KIT; SCO1; FDC; GSFSOW3

P10721

KIT; SCFR

Human Cells

A DNA sequence encoding the extracellular domain of human KIT (P10721-2) (Met1-Thr516) was expressed with the Fc region of human IgG1 at the C-terminus.

Human

The recombinant human KIT/Fc comprises 729 amino acids and has a predicted molecular mass of 82 kDa. The apparent molecular mass of the protein is approximately 93-115 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human KIT-Fc (Cat:503428) at 10 μg/ml (100 μl/well) can bind biotinylated mouse KITL-His (1-189) (Cat:500260), The EC₅₀ of biotinylated mouse KITL-His (1-189) (Cat:500260) is 2.87-6.71 ng/ml.