-
Product Name
Anti-KIT / c-KIT / CD117 antibody
- Documents
-
Description
Rabbit monoclonal to KIT / c-KIT / CD117
-
Tested applications
ELISA, WB
-
Species reactivity
Mouse KIT / c-KIT / CD117
-
Alternative names
Bs antibody; CD117 antibody; c-Kit antibody; Fdc antibody; Gsfsco1 antibody; Gsfsco5 antibody; SCFR antibody; SCFR antibody; SCO1 antibody; SCO5 antibody; Ssm antibody; Tr-kit antibody; W antibody; W antibody; Bs antibody; Fdc antibody; Ssm antibody; SCO1 antibody; SCO5 antibody; SOW3 antibody; CD117 antibody; c-KIT antibody; Tr-kit antibody; Gsfsco1 antibody; Gsfsco5 antibody; Gsfsow3 antibody; PBT antibody; SCFR antibody; C-Kit antibody; CD117 antibody; CD117 antibody; c-KIT antibody; Gsfsow3 antibody; PBT antibody; SOW3 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse KIT / c-KIT / CD117 (rM KIT / c-KIT / CD117; NP_001116205.1; Met1-Thr523).
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB:5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Mouse KIT / c-KIT / CD117.
-
Validations
KIT / c-KIT / CD117 Antibody, Rabbit MAb, Western blot
Immunochemical staining of human RUVBL1 in human testis with rabbit polyclonal antibody (0.02 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.
-
References
- Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
- Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
- Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
- Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
- McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"