Mouse FGFR1/CD331 (His Tag) recombinant protein

Mouse FGFR1/CD331 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ?idiopathic hypogonadotropic hypogonadism? Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.

Fibroblast growth factor receptor 1

FLT2; OGD; FLT-2; HRTFDS; C-FGR; CD331; FLG; KAL2; MFR; CEK; BFGFR; FGFR1; HSPY; FLJ99988; FGFBR; EASK; N-SAM; HBGFR; BFGF-R-1; HH2; AW208770; FGFR-1

P16092

Fgfr1; Flg

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Glu 376) of mouse FGFR1 isoform 1 (P16092-1) was fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse FGFR1 consists of 366 amino acids and has a calculated molecular mass of 40.9 kDa. The apparent molecular mass of rmFGFR1 is approximately 55-65 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts.
The ED₅₀ for this effect is typically 50-200 ng/ml.