Human FGFR1/CD331 (His & GST Tag) recombinant protein

Human FGFR1/CD331 (His & GST Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs) (FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome ?idiopathic hypogonadotropic hypogonadism? Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.

Fibroblast growth factor receptor 1

FGFR-1; N-SAM; HBGFR; HH2; BFGF-R-1; AW208770; EASK; BFGFR; HSPY; FGFR1; FGFBR; FLJ99988; FLG; KAL2; CEK; MFR; HRTFDS; FLT-2; C-FGR; CD331; FLT2; OGD

P11362

FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR

Baculovirus-Insect Cells

A DNA sequence encoding the human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Human

The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 75 KDa band as predicted in SDS-PAGE under reducing conditions.

> 93 % as determined by SDS-PAGE

1. Kinase activity untested
2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:503988) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF acidic (Cat:503100) with a linear range of 15.6-250 ng/ml.
3. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:503988) at 10 μg/ml (100 μl/well) can bind biotinylated human FGF basic (Cat:502055) with a linear range of 0.16-2.5 μg/ml.