Human FGFR1/CD331 (His Tag) recombinant protein

Human FGFR1/CD331 (His Tag) recombinant protein

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Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.

Fibroblast growth factor receptor 1

EASK; FGFR-1; HBGFR; N-SAM; AW208770; HH2; BFGF-R-1; HRTFDS; FLT-2; CD331; C-FGR; FLT2; OGD; HSPY; FGFR1; BFGFR; FLJ99988; FGFBR; KAL2; FLG; CEK; MFR

P11362

FGFR1; BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR

Human Cells

A DNA sequence encoding the human FGFR1 (NP_075594.1) extracellular domain (Met 1-Glu 285) was expressed with C-terminal polyhistidine tag.

Human

The secreted recombinant human FGFR1 consists of 275 amino acids and has a predicted molecular mass of 31 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh FGFR1 monomer is approximately 50-55 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/C 3T3 mouse fibroblasts.
The ED₅₀ for this effect is typically 0.03-0.15 μg/mL.