Mouse FGFR1/CD331 (Fc Tag) recombinant protein

Mouse FGFR1/CD331 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation (By similarity).

Fibroblast growth factor receptor 1

HH2; BFGF-R-1; AW208770; N-SAM; HBGFR; FGFR-1; EASK; MFR; CEK; FLG; KAL2; FGFBR; FLJ99988; BFGFR; HSPY; FGFR1; OGD; FLT2; C-FGR; CD331; HRTFDS; FLT-2

P16092

Fgfr1; Flg

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Glu 376) of mouse FGFR1 isoform 1 (P16092-1) was as fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The secreted recombinant mouse FGFR1/Fc is a disulfide-linked homodimer. The reduced monomer consists of 596 amino acids and has a calculated molecular mass of 66.5 kDa. The apparent molecular mass of rmFGFR1/Fc monomer is approximately 100-110 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts.
The ED₅₀ for this effect is typically 0.1-0.6 ng/ml.