Mouse EPOR/Erythropoietin Receptor (Fc Tag) recombinant protein

Mouse EPOR/Erythropoietin Receptor (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â…  single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

Erythropoietin receptor

EPO R; EPOR; EPO RECEPTOR; ERYTHROPOIETIN RECEPTOR; EPO-R

Q3UTV9

Epor

Baculovirus-Insect Cells

A DNA sequence encoding the mouse EPOR (NP_034279.3) (Met1-Pro249) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

1. Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.05-0.2 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized mouse EPO-His (Cat:504106) at 10μg/mL (100 μL/well) can bind mouse EPOR-Fc. The EC₅₀ of mouse EPOR-Fc is 0.06-0.13μg/mL.